For this study, It was measured the seam puckering based on the mechanical properties of tencel under the proper condition of needlework and machine sewing, analyzed the mechanical properties which are influenced to the seam puckering, and estimated the seam puckering based on the mechanical properties. The results of this study are as follows : There are three types of the seam puckering for each step which is caused by repeated washing and press. Concerning the seam puckering with the number of washing, the more the number of washing is increased, the less the seam puckering is decreased. Concerning the mechanical properties of the sample with the seam puckering, the seam puckering is related to LT positively, B, 2HB, T, W negatively. Among the mechanical properties LT, B, 2HB, T, W are most influenced to the seam puckering. Judging from the result of estimating seam puckering based on mechanical properties, the estimate-formula is satisfied in this study.