The donor organ harvesting technique is an essential component of a successful recipient outcome in organ transplantation. The careful assessment and rapid recovery of deceased donor organs without medical and surgical impairment is the cornerstone for transplant physicians. To expand the donor pool, transplant physicians are continually modifying criteria to accept organs, so-called expanded or marginal donor criteria included: age＞65 years, non-heart beating donor, positive viral serology, split-liver, hypernatremia, prior carcinoma, and fatty liver. The technique of organ harvest has been obvious that a uniform procedure should be developed which is flexible enough to allow the excision of various organ combinations without jeopardy to any of the individual grafts in deceased donor. The rapid infusion method entails no preliminary dissection of the intra-abdominal organs since those organs are cooled by in situ infusion of cold preservation solution and rapid remove in a bloodless field. Avoiding perigraft dissection before cross clamp (warm dissection) contributes to better early graft function than with the warm dissection. The supreme goal of organ harvesting is to ensure that none of the organs are damaged during preparation, perfusion and removal of the organs. (J Korean Soc Transplant 2006;20:14-24)