Pyro-shock generally refers to the severe mechanical transients caused by the detonation of an ordnance device on a structure. Such device on a structure, including linear explosive, and point explosive are widely used to accomplish in-flight separation of structural elements on aerospace vehicle. And they are a significant cause of launch vehicle failures. The launch vehicle being developed in Korea also uses the explosive for separation events. In this paper, the isolator equipped to small satellite launch vehicle made of PTFE(Teflon) is developed to reduce the separation shock. The test to measure dynamic stiffness of PTFE isolator is performed. This test enables us to find the frequency range of PTFE isolator. And,, pyro-shock test using explosive to evaluate the performance of PTFE isolator is executed. From this study, the isolator conformed to frequency range and load requirement is developed using PTFE instead of rubber.