Pseudomonas aeruginosa GRC2, siderophore-producing strain, inhibited growth of Macrophomina phaseolina in vitro and reduced charcoal rot in seeds of Brassica juncea in field when coated with adaptive strains. P. aeruginosa GRC2 and Azotobacter chroococcum AC1 produced indole-3-acetic acid and solubilized insoluble phosphate. A. chroococcum AC1 fixed nitrogen asymbiotically. Urea and diammonium phosphate-adaptive variant strains of P. aeruginosa and A. chroococcum strongly inhibited M. phaseolina in comparison to parental strains. Bacterization of seeds induced seed germination, seedling growth, and enhanced yield of B. juncea by 10.87% as compared to full doses of urea and diammonium phosphate. Both adaptive strains of chemical fertilizers aggressively colonized roots, showing effectiveness to growth and developments of B. juncea.