Human mitochondrial DNA (mtDNA) was analyzed by fluorescence based-polymerase chain reaction-single strand conformation (F-PCR-SSCP) at nucleotide positions between 16261 and 16380 of hypervariable portion I (HV1) in the control region. The best condition of F-PCR-SSCP analysis for mutation separation was identified when the PCR product of mtDNA was electrophoresed on 6% polyacrylamide (49:1 acrylamide:bis) gel containing 8% glycerin at a constant gel temperature of 20℃ for 8 hours. mtDNA SSCP patterns were divided into 7 groups according to mobility shift differentiation of PCR products from 100 Koreans by F-PCR-SSCP analysis, and the groups could be again classified into forty-seven mtDNA SSCP patterns when the mtDNA SSCP patterns were compared with the data of DNA sequencing. When 6 unidentified human remains from a mass disaster were examined family relationships by F-PCR-SSCP analysis, mtDNA SSCP pattern of every human remains was identical with the pattern of mother. so, it was demonstrated F-PCR-SSCP of mtDNA to be very useful technique for individual identification of forensic samples.