Glucan 처리시 에틸렌의 합성 증가가 phenylalanine ammonia-lyase(PAL)의 활성에 미치는 영향과 이때 Ca^2+의 관여 여부에 대해 조사하였다. Glucan은 10 ㎍/mL씩 처리하여 반응을 유도하였다. Glucan 처리시 시간적으로 에틸렌의 합성 최대치는 PAL의 활성이 최대를 보이는 시점보다 선행되었다. 외부에서 ethrel을 처리하여 에틸렌을 발생시킨 경우에도 유사한 결과를 보여 PAL의 양상은 대조구에 비해 20배 가량 활성이 증가하였다. Ca^2+이 첨가되지 않은 배지에서 glucan을 처리한 경우, 에틸렌의 합성은 현저하게 감소하였다. 또한, 에틸렌 생성 효소 억제제인 Co^2-을 glucan과 함께 처리시 PAL의 활성을 감소시켰다. Glucan 처리시 Ca^2-의 영향을 조사하여 본 결과, PAL은 Ca^2+ 억제제인 ethylene glycol-bis(β-aminoethyl ether) N_+N_+N^-_+N^+ - tetraacetic acid (EGTA), verapamil, trifluoperazine 처리시 모든 경우에 활성이 감소하였다. Ca^2+ ionophore인 A23187을 Ca^2+이 첨가된 배지에 처리할 경우 처리 농도가 증가함에 따라 PAL의 활성이 비례적으로 증가됨을 확인하였다. 이상의 결과로부터 glucan 처리시 PAL의 활성 증가는 에틸렌 의존성 반응이며, Ca^2+이 매개하는 것으로 보인다.

Involvement of ethylene and Ca^2+ on the induction of pheaylalanine ammonis-lyase (PAL) was investigated in Daucus carota L. suspension culture system. Ethylene production started to increase about 3 h after glucan treatment. And the maximal induction of ethylene was preceded by PAL induction by 30 min. After the treatment of ethrel, PAL activity was increased. When calls were treated with glucan and Co^2+, PAL activity was simutaneously reduced. Ethylene production was reduced dramatically in calcium-free medium, even though glucan was treated. PAL activity and ethylene production was inhibited conspicuously when ethylene glycolbis(β-aminoethyl ether) N_+N_+N^-_+N^+ - tetraacetic acid (EGTA) was treated with glucan. Verapamil and trifluoperazine also inhibited PAL activity. When cells were treated with calcium ionophore A23187, PAL activity was increased in nontreated medium. We report here PAL activity is increased in related to ethylene production and involvement of Ca^2+ in glucan-treated carrot suspension cells.