Suspension cell lines have been newly established from the calli derived from the immature embryo culture of hexapolid(Triticum aestivum var.sicco), tetrapolid(T.durum) and diploid (T. tauchii or Aegilops squarrosa) wheat species. The chromosomal variation in suspension cultured cell lines was examined and old cell line, C82d, established from T. aestivum var. copain was also used. New method using 1-bromonaphthalene for metaphase trapping of suspension cells was developed. Variation in chromosome number was observed amongst all the suspension lines. Cells with doubled chromosome number and deleted chromosome were also observed. Extensive structural changes in chromosomes were found in C82d line. Chromosome aberrations showed loss of chromosome arms and chromosome segment. The mean chromosome number in suspension cells of T. aestivum var. sicco was 40, in C82d line 33, in T.durum 28 and in T. tauchii 14. The stability of chromosomes in suspension cells of diploid and tetrapolid wheats was higher than that of hexaploid wheat.