Single cells obtained from suspension culture of mature embryo-derived callus in wheat(Triticum aestivum L. cv Jang Kwang) were cultured to regenerate into the plantlet. Cell clusters and embryogenic calluses were efficiently developed from when the single cells cultured on the MS medium supplemented with 10 ㎛ 2,4-D. Upon transfer to hormone-free MS medium containing 10 ㎎/l AgNO_3, embryogenic calluses gave rise to shoots, probably through somatic embryogenesis.