Dunaliella에서 TCA cycle과 g1yoxylate pathway의 활성을 조사한 결과 다음과 같은 결론을 얻었다. 1. cell free homogenate 에서 acetothiokinase, isocitrate dehydrogenase, fumarase, malate dehydrogenase,와 aconitase,의 존재가 인정되었으나, isocitratase, malate synthetase, succinate dehydrogenase 및 oxoglutarate dehydrogenase의 활성은 측정되지 않았다. 2. acetate의 C-1炭素에서 발생되는 CO_2는 C-2炭素에서보다 5倍나 많았으며, succinate는 acetate나 pyruvate와 같은 정도로 호흡에 이용되었다. 3. acetate는 Dunaliella의 생장을 촉진시키지 못했으나, citrate, malate와 succinate 등으로 변화될 수는 있었다. 4. 이상의 결과로 보아 Dunaliella에는TCA cycle은 존재하지만, glyoxylate pathway의 활성은 없는것 같으며, 외부에서 첨가된 acetate는 TCA cycle을 거쳐서 주로 CO_2로 되는 것 같다.

The utilization of acetate by Dunaliella tertiolecta was examined, and the detections and assays of the enzymes of the tricarboxylic acid cycle and the glyoxylate pathway were described. Acetate could not be utilized as a sole carbon source for the growth. The carboxyl carbon of acetate was incorporated more rapidly into CO_2 than the methyl carbon. It was identified that malate, succinate, citrate and etc., were accumulated when〔U-^(14)C〕acetate was supplied to the cell free homogenate. The following enzyme activities were measured; acetothiokinase, isocitrate dehydrogenase, fumarase, malate dehydrogenase and aconitase. Though isocitratase, malate synthetase, succinate dehydrogenase and oxoglutarate dehydrogenase could not be detected, ^(14)C from succinate was easily contributed to CO_2 and cell component. The evidence suggested that the glyoxylate pathway was not operative and showed that the TCA cycle was the all important pathway in the oxidation of acetate to CO_2 in Dunaliella.