Nitrate contamination in the aquatic systems is the primary indicator of poor agricultural management. The influence of sewage sludge application rates (0, 10, 25, 50 and 100 dry Mg/ha) on distribution of nitrate originating from the sewage sludge in soil profiles was investigated. Soil profile monitoring of nitrate was carded out with a Lakeland clay soil in 1997. Irrespectively of the sewage sludge application rates up to 50 dry Mg/ha, the concentration of NO₃-N at the 120 cm depth was below 10 mg/kg and the difference due to the amount of sewage sludge application was negligible at this depth. There was virtually no NO₃-N below 120 cm depth and this was confirmed by a deep sampling up to 300 cm depth. Most of the nitrate remained in the surface 60 cm of the soil. Below 120 cm depth, nitrate concentration was very low because of the deniteifcation even at high sewage sludge rate of 100 dry Mg/ha. The NO₃-N concentrations in the soft fluctuated over the growing season due to plant uptake and denitrificallon. The risk of groundwater contamination by nitrate from sewage sludge application up to high rate of 100 dry Mg/ha was very low in a wheat grown clay soil with high water table (< 3 m).