There was result about biochemistry application of muscles in according to persistent training of aerobic training 1. It is raised about 100％ to produce ATP after aerobic training each part of electronic carrier sequence, and raised (35~100％) active diversity level after aerobic training enezymes in relations citric acid circle, Fatty acid metabolism was raised about 100％ active after aerobic training by particuly Enzymes 2. Mitochondria active CPK was increased two fold after aerobic training and was reacted to increase about 100％ after ketone system enzyme and aerobic trainaing, Mayrate-Aspartate Shuttle`s enzymes was reactived to increase active aerobic training and Glythrow-Faspate shuttle was not reacted to training and the additive Enzymes GTP, ATPase, PK was reacted aerobic training and increased F1 ability to produce ATP. 3, Application to aerobic training was involved to conform fiber during fiber conformation exercise, and it was increased to produce aerobic energy generality reaction to be marrow muscles aerobic training so we was bring to raised ability of exercise using in favor of fatty rather than Hydrocarbon material pertaing trainging muscles exercise, 4, It was not substrate to raise aerobic metabolism, but it was raised 100~150％ to store Glycogen of muscles aerobic training.