The stimulated secretion of golden varnish sap by the infection of microorganisms on the bark of Dendropanax morbifera Lev. was observed in the natural habitats. Four candidate strains of such microorganisms(i.e., Sb1, Sb2, Sb3 and Sb4) were isolated from the bark of D. morbifera which was infected in natural. Of them, Sb3 and Sb4 strains showed stimulated secretion of golden vernish sap with the greatest stimulation by Sb4 strains. The yield of golden varnish sap from the bark of D. morbifera was boosted about 3.4--7.7 times by the inoculation of Sb4 strain in compassion with that collected from lacquer tree(Rhus verniciflua Stokes) by the traditional method. The mycelium color of Sb4 strain was grayish white. The optimum growth temperatures and pH were ranged from 30 to 35℃ and from pH 6.5 to 7.0.