18.97.14.80
18.97.14.80
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희귀 수종 산개나리의 기내 번식
Micropropagation of a Rare Species , Forsythia saxatilis N. through Tissue Culture
문흥규 , 석진영 , 김선창 ( Heung Kyu Moon , Gene Young Suk , Sun Chang Kim )
UCI I410-ECN-0102-2008-520-001456409

Axillary bud explants from 3-year-old seedlings of Forsythia saxatilis N., rare and endangered species in Korea, were cultured on Murashige and Skoog`s medium. The effect of various cytokinins (BAP, kinetin, and zeatin) at the different concentration(0.2, 0.5 and 1.0㎎/ℓ) was tested. Although an apparent shoot proliferation was not observed, zeatin showed slight promotional effect on normal shoot and leaf development. Both shoots and adventive roots could be induced simultaneously when the explants were cultured on the medium with kinetin, but adventive rooting was gradually reduced according as BAP and zeatin concentrations increased. Axillary shoot growth was promoted by the etiolation treatment. Shoot proliferation has been maintained more than three years with consecutive subculture. Rooted plantlets were successfully acclimatized in the artificial soil mixture and showed normal growth after transplantation into field.

[자료제공 : 네이버학술정보]
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