Chlorinated phenolic compounds는 살충제나 생물체에 유해물질로 널리 쓰이고 있는데, 특히 pentachlorophenol(PCP)은 환경의 도처에 존재하는 유독한 물질인 목재 보존재로 주로 쓰이며 EPA에서 priority toxic pollutant로 고시된 난분해성 화합물이다. PCP 분해 세균은 토양에서 분리되었으며 동정 결과 Pseudomonas paucimobilis로 밝혀졌는데, 20 ㎍/㎖의 PCP와 1% glucose를 첨가한 PAS배지에서 26시간만에 기질의 99%이상을 분해하는 것으로 나타났다. 최적온도 및 pH는 각각 30℃, 7.3이며 plasmid curing 시험 결과 PCP를 분해하는 유전자는 plasmid에 존재하는 것으로 밝혀졌으며, 몇가지 항생제에 저항성을 갖는 것으로 나타났다. 또한 SDS-PAGE를 통해 PCP 처리시 유도되는 분자량 42,000, 27,000의 단백질 band를 확인하였다.

Chlorinated phenolic compounds are widely used for pesticide or biocide. Especially, pentachlorophenol (PCP) among chlorinated phenolic compounds is a sort of recalcitrant biocide used primarily for wood preservation, which ubiquitous in the environment, and considered a priority toxic pollutant by the U.S. Environmental Protection Agency (EPA). The PCP degrading bacteria were isolated from the herbicides treated soil and the plants` wastewater by the enrichment culture method and identified as Pseudomonas paucimobilis. When the isolate was inoculated into PAS medium containing 20㎍/㎖ of PCP and 1% glucose, the substrate (PCP) was degraded more than 99% within 26 hours. The optimum temperature and pH are 30℃ and 7.3, respectively. From the plasmid curing test it was known to that PCP degrading genes are located in bacterial plasmid and that the plasmid contained several antibiotic resistant loci in its structure. From the SDS-PAGE experiment it turned out that two bands induced by treatment of PNP were cytoplasmic and periplasmic proteins corresponding to Mw. 27,000 and 42,000, respectively.