휘발유를 sandy loam토양에 농도별로 투여하여 토양미생물군집에 미치는 영향을 조사하였으며 또한 토양에서 탄화수소의 신속한 제거를 위한 bioremediation에 의한 영향을 측정하였다. 휘발유는 토양에서 신속하게 제거되는데 bioremediation 처리에 의한 효과가 거의 없었으며 따라서 제거는 주로 evaporation에 기인하며 생분해는 그다지 기여하지 않았다. 토양에 휘발유가 첨가되었을 때에는 직접세균계수, 균류의 균사길이, 호기성 종속영양세균의 개체수가 2-10배 정도 증가하며 탄화수소 분해세균의 개체수는 최고 100배까지 증가하였다. Bioremediation 처리는 이 수치들을 수배 정도 더욱 증가시켰다. 휘발유를 처리한 토양에서는 fluorescein diacetate 가수분해 활성이 약간 증가하였으나 bioremediation 처리는 더 이상의 활성의 증가를 가져오지 못했다. 토양 탈수소 효소 활성은 bioremediation 처리를 하지 않은 토양에서는 일정한 변화 경향이 없었으나 bioremediation 처리에 의해 활성이 최고 3배 정도 증가하였다.

The effects of gasoline on the microbial community in sandy loam soil were investigated, and the effects of bioremediation which was performed to enhance the removal of gasoline from soil were also measured. Most of gasoline was rapidly dissappeared from soil within several days through evaporation. The effect of bioremediation treatment to enhance the removal of gasoline was negligible. When the soil was contaminated with gasoline, the increasing rates of direct bacterial count, length of fungal hyphae and aerobic heterotroph were in the range of 2 to 10-fold, but the number of hydrocarbon degrader was increased by 2 orders of magnitude. The bioremediation further increased these numbers several times. There was a slight increase of fluorescein diacetate hydrolysis activity in gasoline contaminated soil, but the bioremediation did not increase this activity further. There was no definite pattern of change in soil dehydrogenase activity in untreated soil, but the bioremediation increased this activity up to 3 -fold.