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KCI 후보
치주염환자의 Porphyromonas gingivalis 열충격단백에 대한 T-세포 항원 결정부위 특이성 연구
T-cell epitope specificity for Porphyromonas gingivalis heat shock protein in periodontitis
이지영 ( Lee Ji Yeong ) , 이주연 ( Lee Ju Yeon ) , 최점일 ( Choe Jeom Il )
UCI I410-ECN-0102-2009-510-002684307
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Due to considerably high degree of sequence homology between bacterial and human heat shock proteins(hsp), it has been widely thought that this protein might be involved in autoimmune disease mechanisms in humans. To elucidate how stress proteins contribute in the immunopathogenesis of periodontitis, the present study was performed to evaluate the T cell immune responses specific to Porphyromonas gingivalis (P. gingivalis) heat shock protein (hsp)60 and T-cell epitope specificities for P. gingivalis hsp60 in periodontitis. Anti-P. gingivalis IgG antibody titers were elevated in all patients. We could establish P. gingivalis hsp-specific T cell ines from the peripheral blood of peridontitis, a mixture of CD4^(+) and CD8^(+) cells. Of 108 overlapping synthetic peptides spanning whole P. gingivalis hsp60 moleculc. ten peptides with epitopes specifities for T-cell were showed. Interestingly, ten epitopes were also identified as T-cell epitopes in the present study as well as B-cell epitopes in peridontitis. Therefore, all the ten representative epitopes were designated as common T-and B-cell epitopes for peridontitis. It is critical in developing a peptide vaccine strategy for potential prevention of periodontitis. It was concluded that P. gingivalis hsp60 might be involved in the immunoregulatory process of periodontitis with heat shock protein specificities.

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