The vortex tube is a simple device for separating a compressed gaseous fluid stream into two flows of high and low temperature without any chemical reactions. Recently, vortex tube is widely used to local cooler of industrial equipments and air supply system. The phenomena of energy separation through the vortex tube was investigated experimentally. This study is focused on the effect of the diameter of cold end orifice diameter on the energy separation. The experiment was carried out with various cold end orifice diameter ratio from 0.22 to 0.78 for different input pressure and cold air flow ratio. The experimental results were indicated that there are an optimum diameter of cold end orifice for the best cooling performance. The maximum cold air temperature difference was appeared when the diameter ratio of the cold end orifice was 0.5. The maximum cooling capacity was obtained when the diameter ratio of the cold end orifice was 0.6 and cold air flow ratio was 0.7.