The MeOH extracts obtained from the flower petals of Carthamus tinctorius were solvent-fractionated with EtOAc, n-BuOH, and H₂O, successively. From the n-BuOH exact 2 flavonoid compounds were isolated through the repeated silica gel column chromatographies. From not only the results of physico-chemical data including HMBC but also the adaptation of acid hydrolysis, the chemical structures of the compounds were determined as 3-Ο-[β-D-glucopyranosyl (1→2) β-D-glucopyranosyl] kaempferol and 3-Ο-[α-L-rhamnopyranosyl (1→6) β-D-glucopyranosyl] kaempferol. The compounds exhibited IC_(50) values in Grab2-Shc activity to be 43 and 47 ㎍/㎖, respectively.