In this study, the bacteria which could hydrolyze the fibrin produced through the blood coagulation mechanism in the human body, were isolated from Chungkookjang. The KCK-7 strain was selected among the isolated bacteria as the best strain for fibrinolytic activity. It was spore forming and Gram positive. C_(15-0) anteisa fatty acid and C_(15-0) iso fatty acid were 40.85% and 19.47%, respectively as major component among its cellular fatty acid composition. It showed the similarity of 63.6%, compared with standard strain. It was thus identified to be Bacillus subtilis according to Bergey`s manual of systematic bacteriology and its fatty acid profiles af Gas chromatography. The optimum culture temperature and pH were 37℃ and 8 for the production of fibrinolytic enzyme by Bacillus subtilis KCK-7.