This study was carried out to investigate the genetic characteristics and specitic genetic marker of Han Woo cattle by random amplified polymorphic DNAs(RAPD) and restriction fragment length polymorphism(RFLP) using the PCR method. According to the results of RAPD using 48 kinds of primer, band patterns were almost same regardless of cattle breeds. However, some particular patterns were dected only from Han Woo cattle. The band patterns were not afFected by the condition of PCR. Therefore, it can be speculated that the band patterns can be employed as a specific marker for Han Woo cattle. After extraction of genomic DNA from 20 Han Woo cattle and 21 Holsteins, the second exon of BOLA-DRB3 gene was amplified by specific primer by PCR. And then after cutting these amplified PCR products by three kinds of restriction enzyme RFLP patterns were investigated. Interestingly, we could detect two novel BOLA-DRB3 genotypes, which has not been reported so far. The newly detected genotypes were designated as BOLA-DRB3 kor 1 and BOLA-DRB3 kor 2. Therefore, it can be considered that BOLA-DRB3 kor 1 and BOLA-DRB3 kor 2 could be utilized as a specific markers which can distinguish Han Woo cattle from the other breeds.