In this study, removal characteristics of nutrient were evaluated using new developed multi-porous polyurethane media(BIOCUBE) with synthetic wastewater and leather industry wastewater as influents. According to the lab. test using synthetic wastewater as influent, to select the best suitable media, removal characteristics with media size and pore size were estimated. Then removal efficiencies of nutrients based on the organic and the ammonia load were tested. For the test of application of industrial wastewater, removal characteristics of organics and nitrogens by introducing leather industry wastewater were evaluated. As regards test with pore size and media size, 40ppi media were better than 20ppi media and 10㎜×10㎜×10㎜ were about 2 times as high as 20㎜×20㎜×20㎜. As test by synthetic wastewater, organic removal efficiencies of BIOCUBE process were 2.5∼3 times as high as those of conventional activated sludge process and nitrification rates were 3∼4 times. Introducing by leather industry wastewater, organic removal efficiencies and nitrification rates were so high but denitrification rates were so low because of lack of carbon sources.