Age and growth of three-lined tonguefish (Cynoglossus abbreviatus) were studied using samples from the waters off Yosu, Korea, from June to December, 2001. Sagittal otoliths had relatively clear annuli. Each annulus was formed once a year in April. The peak of the gonadosomatic index occurred also in April. The oldest fish observed in this study was 5 years old for females and 4 years old for males. The relationship between the otolith radius (R) and total length (L) was as follows: L=14.921R-2.5318 for females and L=13.527R- 0.5584 for males. The relationship between total length and body weight (W) was as follows: W=0.0008L^(3.54) for females and W=0.0029L^(3.14), for males. The growth in length of the fish was expressed by the von Bertalanffy`s growth equation as: L_(t)＝44.54(1-e^(0.16(t+2.69))) for females and L_(t)=41.52(l-e^(0.13(t+3.34) ) for males.