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KCI 후보
L-lysine에 의한 Microcystis sp.의 선택적 성장억제
The Effect of L-lysine on Growth Inhibition of Microcystis sp.
한명수 ( Han Myeong Su ) , 최영길 ( Choe Yeong Gil ) , 송석환 ( Song Seog Hwan ) , 신규철 ( Sin Gyu Cheol )
UCI I410-ECN-0102-2009-470-003398709

본 연구는 L-lysine(l-2, 6-Diaminohexanoic acid)의 영향을 받은 남조류와 남조류에 영향을 주는 L-lysine의 농도를 파악 하고자 20종의 Microcystis sp.를 실험에 사용하였다. 남조류의 생장 억제 능력은 double-layered agar method와 microplate method를 이용하여 측정하였다. L-lysine의 농도를 100㎍ ml^(-1)~300㎍ ml^(1) 이상에서 처리한 경우 Microcystis ichthyoblabe NIER-10021 외 7종 Microcystis sp.에서 투명대가 형성되었다. Microplate method에서는 Microcystis viridis NIER-10020외 7종의 Microcystis sp. 에서는 10~500㎍ ml^(-1)의 농도에서 생장억제 및 lysis를 나타내었다. 그리고 Microcystis viridis NIER-10020 외 3종은 10㎍ ml^(-1)이하의 낮은 농도에서도 생장억제 및 분해를 나타내는 높은 활성을 보였다.

Various physico-chemical and biological methods have been used to remove cyanobacteria which causes blooms and releases toxin. The purpose of the following experiment is aimed finding out which cyanohacteria are affected by L-lysine and what concentration of L-iysine inhibits cyauobacteria. The 20 samples of Microcystis sp. have been tested. To prove the growth inhibition on Microcystis sp., double-layered agar method and microplate method have been used. When the Concentration of L- lysine is as heavy as 100 ㎍ ml^(-1)-300 ㎍ ml^(-1) some Microcystis sp. have made halo zone. Some Microcystis sp. have shown so high activity as to he inhibited in their growth by the L-lysiue of concentration 10 ㎍ ml^(-1) with microplate method. These activities are various in accordance with every species. In additions, the microplate method has been proven to he an easy way which examine the lytic activity on the species of algae.

[자료제공 : 네이버학술정보]
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