한국산 인삼을 60% acetone으로 추출하여 Sepadex LH-20 gel column chromatography, MCI-CHP 20 gel column chromatography, μ-Bondapack C_(18) gel column chromatography을 이용하여 polyphenol 분획물을 3종류를 분리하여 항산화 효과, phospholipase A₂저해효과 및 암세포 증식억제 효과를 검토하였다. 항산화 효과에서 분획물 Ⅱ가 200ppm에서 35.17%의 라디칼소거능을 보였다. 분획물 Ⅲ는 Fe^(2+), OH가 존재 시 150ppm에서 48.49%, 25%의 항산화효과를 나타내었다. Phospholipase A₂저해활성은 분획물 Ⅲ이 60㎍/㎖에서 48.9%의 활성을 나타냈다. HT 29 cell에 대한 인삼의 세포 독성은 분획물 Ⅱ에서 0.25㎎/㎖에서 73.29%의 가장 높은 억제능을 나타내었다.

The polyphenol fractions of Korean ginseng were purified using Sephadex LH-20, MCI gel, Bondapak C_(18), TLC, and HPLC from the 60% acetone soluble fraction. Fraction I showed 48.16%, 79.71% and 43.55% inhibition at 150 ppm against lipid oxidation in the presence of copper ion, superoxide and hydrogen peroxidation. Electron donating abilities of fraction Ⅱ showed 35.17% inhibition at 200 ppm. Fraction Ⅲ showed 48.49% and 25% inhibition at 150 ppm against lipid oxidation in the presence of ferrous ion and hydroxy radical ion. The phospholipase A₂ inhibitory effect of fraction Ⅲ was 48.9% at the concentration of 60 ㎍/m/. The cytotoxic effects of fraction Ⅱ was the highest (73.29% at 0.25 ㎎/m/) among the tested polyphenol fractions.