양하(Zingiber mioga Roscoe) 근경의 에탄올 추출물을 재료로 Staphylococcus aureus, Bacillus subtilis 및 B. cereus에 대한 activity-guided fractionation을 실시하여 항균성 물질 세 가지를 얻었다. 항균력 실험 결과, 화합물Ⅰ과 Ⅲ은 세 가지 균주 모두에 활성이 있었고, 화합물 Ⅱ에서는 B. subtilis과 B. cereus에서만 활성이 나타났다. 그 중에서 가장 활성이 강한 화합물Ⅰ을 Bioscreen C로 optical density(600㎚)를 측정하여 증식억제실험을 한 결과 10ppm 처리시 B. subtilis과 B. cereus에서 72시간동안 강한 증식억제효과를 나타내었으며, S. aureus에서는 25ppm 처리 시 72시간동안 완전증식억제효과를 나타내었다. ^(1)H-NMR, ^(13)C-NMR, DEPT, ^(1)H-^(1)H COSY, HMQC, HMBC 및 IR 스펙트럼 등을 분석한 결과 화합물 Ⅰ, Ⅱ, Ⅲ은 labdane type diterpene인 (E)-8β(17)-epoxylabd-12-ene-15,16-dial(C_(20)H_(30)O₃, MW=318), galanolactone(C_(20)H_(30)O₃, MW=318) 그리고 galanal A(C_(20)H_(30)O₃, MW=318)로 각각 동정되었으며, 이들은 양하의 근경에서는 처음 분리된 것이다.

In order to isolate antibacterial substances from the rhizome of Zingiber mioga Roscoe, the ethanol extracts was fractionated according to the activity against Bacillus subtilis B. cereus and Staphylococcus aureus. Three antibacterial substances were isolated and purified by column chromatography and recrystallization. Compounds Ⅰ and Ⅲ showed activity against all the tested bacterias and compound Ⅱ exhibited the activity against B. subtilis and B. cereus except S. aureus. Compound I was examined antimicrobial activity aginst B. .subtilis, B. cereus and S. aureus by optical density using Bioscreen C Compound I showed strong growth inhibition at 10 ppm on B. subtilis and B. cereus for 72 hrs, and at 25 ppm on s. aureus. On the basis of spectrometric studies including ¹H-NMR, ^(13)C-NMR, DEPT, 1H-1H COSY, HMQC, HMBC and IR, compounds Ⅰ, Ⅱ and Ⅲ were identified ai (E)-8β(17)-epoxylabd-12-ene-15,16-dial (C_(20)H_(30)O₃, MW=318), galanolactone (C_(20)H_(30)O₃, MW=318) and galanal A(C_(20)H_(30)O₃, MW=318), respectively. These results are the first reports on the isolation (E)-8β(17)-epoxylabd-12-ene-15,16-dial, galanolactone and galanal A from the rhizome of Zingiber mioga.