Using the high-resolution banding technique, a karyotyping for Korean Cattle was performed. Blood samples were collected from 119 male cattle that had been stationed at the National Livestock Research Institute for progeny test and then were prepared to in vitro cultures followed by karyotyping; morphology and G-, Cbanding patterns of prophase, prometaphase and metaphase chromosomes were analyzed. All their 58 autosomes revealed almost a zero value of centromeric index, indicating that they are acrocentric; sex chromosomes X and Y were submetacentric and metacentric chromosome, respectively. Chromatin condensation, which increased with the progression from prophase to metaphase in all chromosomes, was greater in longer chromosomes than in shorter ones. Prophase and metaphase chromosomes exhibited 544 and 303 G-bands per haploid, respectively, with variable band intensity. This number of G-bands in prophase was much greater than G-band that can be obtained by a conventional method, implicating an improved karyotyping for Korean Cattle. Moreover, autosomes exhibited the C-bands at or near centromere, whereas sex chromosomes had variable numbers of them on variable locations. The proportion of constitutive heterochromatin, ranging 13∼27%, increased with decreasing chromosomal length and also with increasing chromatin condensation. We propose these results as new standard karyotyping for Korean Cattle