Estimation of inbreeding and relationship coefficients using DNA fingerprinting in the Korean Cattle, revealed that the inbreeding coefficient dependent on band frequency in Korean Cattle(0.100) was lower at least 10% compared to foreign cattle breeds [Holstein(0.338), Charolais(0.217), Aberdeen-Angus(0.283), and Simmental(0.160)). Band sharing value of relationship coefficient groups(0.5, 0.25, 0.125, 0.0625) in Korean Cattle were 0.704, 0.611, 0.463, and 0.296 respectively. Genetic similarity has increased in group of higher relationship coefficients. Relationship coefficient(Y) on genetic similarity(X) could be accurately estimated as linear regression of Y = 0.526X + 0.469. Furthermore, the results will be useful to estimate genetic parameters of animal population without pedigree.