The objectives of the present study were to investigate 1) effects of various extraction solution on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) patterns of muscle proteins, 2) species-specific muscle protein components among beef; pork, and chicken meat and 3) hexose amounts and lectin staining patterns of muscle proteins from Hanwoo and Holstein beef. Although great amount of approximately 1.1 kDa and 7.5 kDa protein components were extracted from Hanwoo beef with 0.5% sodium dodecyl sulfate (SDS) or saline solution and Triton X-100, respetively, there were no differences in SDS-PAGE patterns of muscle proteins from Hanwoo or Holstein beef which were extracted with distilled water, 0.5% SDS, 0.5% Triton X-100 or saline solution. The SDS-PAGE patterns of muscle proteins from various parts of Hanwoo beef did not differ from those of Holstein beef. However, SDS-PAGE patterns of muscle proteins among beef, pork and chicken meat differ significantly. However those of 68, 47, 43 and 28 kDa were observed particularly in beef but not in pork or chicken meat. However those of 40 and 34 kDa were identified in pork but not in beef or chicken meat. The components of 50 and 38 kDa were identified in chicken meat but not in beef or pork. Observations that amount of hexose at various parts of Hanwoo and Holstein beef did differ in a certain degree, and that lectin staining patterns[in particular, affinities for Dolichos diflorus agglutinin (DBA) and Ulex europaeus agglutinin (UEA-1)]did differ between Hanwoo and Holstein beef suggest that unique sugar chain may be synthesized in breed-specific manners in beef.