This study examined the pattern of in vitro development of quail embryos cultured in surrogate egg-shells and investigated an in vitro culture system of quail embryos to provide opportunities for generic manipulation of quails. To investigate die pattern of in viro development of quail embryos in surrogate egg-shells, 2-day-old quail embryos were implanted into surrogate egg-shells, sealed with wrap and cultured at 37.6℃ with relative humidity of 65.5±5%. Quail embryos in the blasfodermal stage were implanted into surrogate quail and chicken egg-shells sequentially, sealed with wrap, and cultured at 37.6℃ with the relative humidity of 65.5±5%. An average length of the third toe of quail embryos cultured in surrogate egg-shells at 15 days of incubation was 12.49㎜, and was 13.03㎜ for the control culture. An average dry weight of quail embryos cultured in surrogate egg-shells at 15 days of incubation was 1.216g, and was 1.042g for the control culture. When blastodermal embryos were cultured in surrogate egg-shells of a chicken, the average hatchabilities were 11.8% for wild type and 24.6% for black type. When two-day-old embryos were cultured in surrogate egg-shells of the chicken, average hatchabilities were 55.6% for wild type and 51.3% for black type, and when blastodermal embryos were cultured in surrogate eggshells of quail from the blastodermal stage to 2-days-old, and thereafter to hatching were recultured in surrogate egg-shells of chickens, average hatchabilities were 36.0% for wild type and 51.9% for black type. The results for our culture system indicated that it would be practical for the production of transgenic birds.