This study was conducted to examine the effect of difference in materials of aerobic wear on both human body`s physiological reactions and subjective wearing sensation by comparing and analysing not only cotton spandex A, B and C but nylon spandex D, E and F used as actually wearing materials. The rectal temperature of type A and E remained at high degree, the type C was higher from after high-speed running but dropped sharply when taking a break finally. Both skin temperature and mean skin temperature dropped sharply due to sweat occurred during physical exercise,and then rose slowly when taking a break. Type A -D and B-E showed that the mean skin temperature remained at high degree when wearing a cotton spandex. Type C`s temperature within its aerobic wear was lower than type F while its relative humidity was higher than type F. Wearing sensation showed a change similar to wearing, particularly, which was remarkable in type B-E. Also, it was shown that humidity sensation, tactile sensation and comfort sensation were good when wearing the cotton spandex.