Denims were treated with neutral cellulase, acid cellulase, stone, and stone-neutral cellulase respectively at different cellulase concentrations varying treatment times in rotary washer. The effect of washing on denim was estimated by the changes in weight, color, back staining, tear strength, flex stiffness, and surface characteristics. Also the comparison of neutral cellulase, acid cellulase, stone, and stone-neutral cellulase washings was studied. Washing removes surface fibers and provides weight loss, color difference, back staining, and a decrease in tear strength and in flex stiffness. Stone-neutral cellulase washing and acid cellulase washing have a larger washing effect than neutral cellulase washing and stone washing.