The in vitro predictive tests in cancer chemotherapy of cancer cell lines to anticancer drugs were determined using novel dye exclusion assay ［NDEA］, [3H] thymidine incorporation, and clonogenic assay [.CA＞. Antitumor effect of Bleomycin, Cis-platin, Vinblastine, Methotrexate to HEp-2, BI6 cell lines using rapid assays was compared with [CA> in this study. In dye exclusion assay of B l6 cell line, cancer cells were sensitive to Bleomycin at all concentrations, to Vinblastine at the level of peak plasma concentration ［PPC］×l/10 [PPC](P<0.05). And Bleomycin revealed relatively good cytotoxicity than that of CDDP and vinblastine at ×10［PPC］, (P<0.05). HEp-2 cells were resistive to methotrexate at the level of ×l00［PPC］ (P<0.05) In [3H] thymidine incorporation assay, B 16 cells were sensitive to Bleomycin, CDDP, Vinblastine at the level of ［PPC］×10 ［PPC］(P<0.01). Dose-dependent drugs of bleomycin, CDDP were more sensitive than Vinblastine at high concentration (P <0.05). In clonogenic assay, HEp-2 cell line was sensitive to three drugs of all concentrations except ×1/10 ［PPC］ of CDDP. B 16 cell line was sensitive to all drugs(P<0,01). In comparison of chemosensitivity tests among three assays, the results were correlated(γ=0.99, P<0.05).