Under Light and Scanning electron microscope, the pupal morphology of mulberry silkworm Bombyx mori Linn. revealed the reduced prothorax and metathorax, well developed mesothorax, less defined last pair of spiracle, well exposed prothoracic femora and wing pads approaching the anterior margin of Ab III. The important sex separating characters viz. pupal size, weigt, antennal elevations, intersegmental lines and genital openings have been discussed. Further, two separate openings bursa copultrix and ovipositional opening were observed, performing different functions in adult moth.