This study was carried out to improve the liquid culture methods of paecilomyces haponica. The results show that the size of granular mycelium is smaller when the shaking speed is increased. Especially, the granular mycelium is the smallest at the shaking speed of 150 rpm under the photoperiod of 12L-l2D. Dry weight of mycelium was averagely 1.216g in the Silkworm larva (SL) medium, and the weight was 2 times heavier than in the Potato dextrose (PD) medium. By adding 6g of 6 mm beads in the SL medium, the dry weight is increased to 1.332g. The optimal addition of silkworm larval powder to the culture medium for best harvest was 1.360±0.67g in dry weight.