To study the non-sprouting causes of mulberry saplings, 8 treatments, borax lime compost, borax, lime, Jamsi fertilizer No. 1, heavy nitrogen application, blanch harvest, heavy harvest and no treatment were applied on the nursery garden. The tract used as the paddy field before sapling produced was very poor in the soil chemical and physical properties. The results were as follows; 1. The survial rates of saplings were higher than 98% in the treatments of borax, lime, Jamsi fertilifer No. 1, whereas heavy nitrogen 59% and heavy harvest 71%. 2. The survial rate was showed deeper relationship with the chemical concentration in stem cortex than in root cortex, especially the content of B, Cu and Zn. 3. The nitrogen content in stem cortex was much higher in the heavy nitrogen treatment by 0.89%, where as 0.6∼().7% in the other treatments. However, treatments of the heavy harvest and the branch harvest were around 5%. 4. In order to decrease the non-sprouting sapling rate, nursery garden should be good in soil chemical and physical properties. Harvest from saplings and heavy nitrogen application should be prevented and lime or Jamsi fertilizer should be applied to saplings.