Flower buds of the mulberry (Morus alba L., Morus bombycis Koidz.) were cultured under different condition such as basal media. And various concentrations of plant growth substances. Effects of the culture condition on growth of the buds and organ regeneration were investigated and the results obtained are as follows: Murashige and Skoog(M.S) medium was more effective on budding and growth of female (Keomseolppong) and male (Kaeryangppong) flower buds isolated directly from branches, compared to Greshoff & Doy(G.D) and Wolter & Skoog(W.S.) media. The growth of the female buds was promoted at higher concentration of benzyl amino purine(BAP) i.e., 2.0ppm. The female and male buds cultured after cutting for seven days showed better growth than those without cutting treatment. The females and the males bloomed to form healthy stigmas and anthers, respectively, when cultured on M.S media containing high Kinetin with low concentration of indole acetic acid(IAA).