본 연구는 단백질 분해효소 저해물질을 탐색하고자 방선균을 선택적으로 분리할 수 있는 colloidal chitin 고체배지를 사용하여 강원도 원주 일원의 토양에서 Streptomyces sp. SK-862를 선별하여 배양여액으로부터 단백질 분해효소 저해물질을 분리하였다. 최적의 배양온도와 초기 pH는 각각 28℃와 8.5이었다. 가장 적합한 배지조성은 1.5% glucose, 0.5% peptone, 0.1% K_2HPO_4, 0.05% CaCO_3이었으며, 60시간 동안 진탕 배양하였을 때 저해물질 생산이 최대가 되었다.

A inhibitor acting on substrate proteolytic enzyme was isolated from culture broth of Streptomyces sp. SK-862, which had been isolated from soil in Wonju City, by using the colloidal chitin agar medium. The optimum culture temperature and initial pH for the production of the protease inhibitor was 28℃ and pH 8.5, respectively. The optimum culture medium was composed of 1.5% glucose, 0.5% peptone, 0.1% K_2HPO_4, 0.05% CaCO_3 and initial pH 8.5. The inhibitor production was maximum when the strain was incubated in shaking incubator at 70 strokes for 60 hours.