Ginsenosides Rh1 and Rh2 induced the differentiation of F9 teratocarcinoma stem cells. These agents are structurally similar to the steroid hormones, therefore, we speculated that the steroid receptor (s) or novel nuclear receptor (s) could be involved in the differentiation process induced by them. Based on this speculation, we tried to clone new nuclear receptors with reverse transcription-polymerase chain reaction (RT-PCR) method by isolating RNA from F9 teratocarcinoma cells induced by ginsenosides. By using RT-PCR with degenerated primers from highly conserved DNA binding domain of nuclear receptors, we identified several nuclear receptors. In northern blot analysis we found that these clones are transcriptionally regulated by ginsenoside Rh1 or Rh2 treatment. Further characterizations of these clones are needed to identify the mechanism of gene expression which has an important role in the differentiation of F9 cells induced by ginsenosides.