본 연구는 streptozotocin으로 유발된 고혈당 당뇨병 쥐에대한 고려인삼의 지용성분획의 혈당강하작용을 혈액성분과 간의 당, 지질대사 관련 효소의 활성을 분석하여 추구하였다. 흰쥐(Sprague Dawley, 170∼200g, ♂)에게 몸무게㎏당 70㎎의 streptozotocin을 복강 투여하고 7일간 정상사료로 사육한 후 혈당치가 340㎎/100㎖(340∼420㎎/100㎖)이상인 쥐에게 한마리당 매일 5∼20㎎의 인삼 지용성분획을 3일간 복강투여하고 최후 투여시부터 16시간 질식시킨 후 혈액과 간을 채취하여 혈액성분과 간 효소의 활성을 측정하고 대조군과 비교하였다. Streptozotocin 투여로 크게 상승된 혈액의 glucose, ketone체, 유리지방산 양이 인삼 지용성분획 투여로 인해 유의적으로 강하되었으나 투여량에 따른 혈당 강하 작용에는 큰 차이가 관찰되지 않았다. 또한 streptozotion 투여로 강하된 쥐 간의 glucokinase, phosphofructokinase, pyruvate kinase, 6-phosphogluconate dehydrogenase와 acetyl CoA carboxylase의 활성도 유의적으로 개선되었으며 인삼 ginsenoside의 개선효과와 비교할만한 효과가 있었음이 관찰되었다. 미국인삼과 중국과 인삼의 지용성분획의 혈당강하 작용을 비교한 결과 큰 차이는 없었으며 인삼의 지용성분획의 혈당강하 작용에 대한 연구는 앞으로 좋은 연구과제일 것으로 생각된다.

This study was made to understand a hypoglycemic action of the fat soluble fraction of Panax ginseng C.A. Meyer in streptozotocin induced diabetic rats by determining the activities of several enzymes related to carbohydrate and lipid metabolism as well as several blood component levels such as glucose and ketone bodies, and non-esterified fatty acids. Albino rats (Sprague Dawley, 170∼200 g, ♂) were injected once with 70㎎ streptozotocin/㎏ body weight intraperitoneally and fed with ordinary diet for 7 days, and then the fat soluble fraction (5㎎∼20㎎/day/rat) was injected intraperitoneally once a day for three days to rats having high blood glucose level over 340㎎/100㎖. After a final injection of the fat soluble fraction, rats were starved for 16 hours followed by the analysis of blood serum and liver enzymes. It was found that increased levels of glucos, ketone bodies and free fatty acids in screptozotocin induced fats were decreased appreciably by administration of the fat soluble fraction. However, the amount of administered fat soluble fraction did not show any significantly different hypoglycemic action. Decreased activities of glucokinase, phosphofructokinase, pyruvate kinase, 6-phos-phogluconate dehydrogenase and acetyl CoA carboxylase of the liver of streptozotocin induced diabetic rats were greatly modified suggesting that a hypoglycemic action of the fat soluble fraction was also appreciable as ginseng saponin fraction. We also compared a hypoglycemic action of the fat soluble fraction prepared from American ginseng and Chinese ginseng with that of Korean panax ginseng. No significant difference of the hypoglycemic activity was observed between the above ginseng fat soluble fractions, suggesting that a study of the fat soluble fraction might be one of the most interesting subjects relating to diabetic hyperglycemia in the near future.