Lysosome의 안정성에 미치는 인삼사포닌의 영향을 관찰하기 위하여 AP활성을 조사하고 이의 기작을 규명하고자 하였다. 저장성 용액이 상층액에서 AP활성이 등장성에 비해 크게 증가되었으며 인삼사포닌 처리에 무관하였다. 등장성 용액에서 AP활성이 10^-6, 10^-5와 10^-4의 인삼사포닌 농도에서는 감소되었으나 10^-2와 10^-1%에서는 증가되었으며, 강력한 저삼투성 충격을 가했을 AP활성은 인삼사포닌 처리에 무관하였다. ATP를 첨가하면 AP활성이 감소되었다. 이상의 결과로 보아 인삼사포닌은 일단 유리된 AP에는 영향을 주지 않고 10^-6, 10^-5와 10^-4의 농도에서는 lysosome의 안정성을 증가시킨다고 사료된다.

The effect of ginseng saponin on acid phosphatase(AP) activity in liver lysosomes was investigated and the mechanism by which ginseng saponin may function on the integrity of lysosomes was discussed. The experimental results obtained are summarized as follows; 1. A very marked increase in the AP activity was observed in the supernatant of hypotonic medium, as compared with that of isotonic medium, indicating that the hypoosmotic shock per se results in activation through osmotic lysis of particles. 2. Ginseng saponin had no effect on the activity of AP if once released from lysosomes when lysed in the hypotonic medium, suggesting that ginseng saponin has no effect on the enzyme molecules per se. 3. The AP activity in isotonic medium suspensions was decreased at the concentrations fo 10^-6, 10^-5 and 10^-4% of ginseng saponin, but increased at 10^-2 and 10^-1%. It`s suggested that ginseng saponin enhances the integrity of lysosomes at 10^-6, 10^-5 and 10^-4%, but decreases it at 10^-2 and 10^-1%. 4. Suspending particles in distilled water resulted in no correlation of AP activity with treatment with ginseng saponin. 5. The AP activity was decreased in the presence of ATP, showing the possible significance of ATP as a lysosomal stabilizer and the possibility that ginseng saponin may effect a membrane bound ATPase system by which lysosomal AP release may be controlled.