남조류의 한 종인 Anabaena flos-aquae를 대상으로 Live/Dead BacLight Viability kit(Molecular Probes Co.)를 이용하여 세포사멸계수를 계산하였다. Live/Dead BacLight kit를 Anabaena flos-aquae에 가하면 살아있어 세포막이 정상인 세포에서는 녹색의 형광을, 죽어서 세포막이 손상된 세포에서는 적색의 형광이 발하였으며, 배경은 전혀 형광을 발하지 않았다. 인위적으로 죽은 세포와 산 세포의 비율을 조절하여 준비된 표본에 BacLight kit를 가하여 녹색과 적색의 형광을 발하는 세포의 수를 측정한 결과 처음에 준비된 살아있는 세포와 죽은 세포와의 비율과 같았으며 이것은 fluorescence emission 측정으로 확인되었다. 적용 가능성을 확인한 후에 인산염을 제한시킨 Anabaena flos-aquae의 chemostal culture에서 질소를 고정하는 경우 성장률이 0.3/일에서는 죽은 세포의 비율이 2.6%이었고, 0.7/일에서는 1.2%로 성장률이 증가함에 따라 죽은 세포의 비율은 감소하였으며, 세포사멸계수는 0.008이었다. 질산염을 이용하는 환경에서는 성장률이 0.3/일에서 4.3%이었고, 0.7/일에서는 1.6%로 같은 경향을 나타내었다.

The measurement of cell death constant in Anabaena flos-aquae was tested by the Live/Dead BscLight Viability kit(Molecular Probes Co., Seatle, WA). When the Live/Dead BacLight Viability kit was applied to Anabaena flos-aquae, the cells with intact cell membrances(live cells) stained fluorescent green, while the cell with damaged membranes(dead cells) stained fluorescent red and the background remained virtually nonfluorescent. The ratios of live : dead cells in the cell suspension were controlled artifically and Live/Dead BacLight Viability kit was applied to them. The ratios of green:red fluorescent cells in the cell suspension were the same as those of live : dead cells controlled artifically. It was also approved by the fluorescence emission. The cell death constant was measured in the P-limited Anabaen flos-aquae chemostat culture in the N-fixing and KNO_3-supplied conditions. The culture in N-fixing chemostat had a dead cell proportion of 1.2% at the growth rate of 0.7/day and increased to 2.6% at the growth rate of 0.3/day. The cell death constant of N-fixing culture was 0.O08/day. There was a same trend in the KNO_3-supplied chemostat culture. The proportion of dead cell was 1.6% of dead cell proportion at the growth rate of 0.7/day and increased to 4.3% at the growth rate of 0.3/day.