Using natural frequency measurement method, which is one of NDT method, natural frequency of the hot-rolled mild steel plate(specimen) under fatigue loading was measured. Between the degradation of the specimen under fatigue loading and the variation of the natural frequency of the specimen was investigated. As a result, the degradation of the specimen was described and monitored as variation of natural frequency of specimen. The natural frequency of specimen decreased gradually under fatigue loading. This means the variation of material properties of specimen. Especially. It means the variation of Young`s modulus of specimen.