High Density Lipoprotein has led to large number of observations regarding factors which influence their concentration. HDL are separated into 3 subfractions, HDL, HDL2 and HDL3, by ultracentrifugation methods. Total cholesterol, HDL cholesterol and HDL2, HDL3 cholesterol were analysed in the serum of patients with chronic persistent hepatitis, chronic active hepatitis, liver cirrhosis, hepatoma, acute viral hepatitis, fatty liver, and coronary artery disease. Other parameters for liver dysfuction also were studied. The results ivere as follows. 1) The mean level of cholesterol in normal control group were 157.3+12.6 mg/dl in male, 152.3+ 14. 5 mg/dl in female group. In various liver diseases, there were no significant difference among the groups except liver cirrhosis (p<0.05). 2) There were significant decrease in the level of total HDL in liver cirrhosis and hepatoma (p < 0.05). 3) The level of HDL2, in normal control group were 13.52+3.84 mg/dl in male and 17.28+6.38 in female. There were significant decrease in the level of HDL2, in patients with chronic active hepatitis (p<0.05), liver cirrhosis (p<0.01), and hepatoma (p<0.01). 4) There were no significant difference in the level of HDL3, among the groups. 5) There were significant decrease in the ratio of HDL2/HDL3 in patients with chronic active hepatitis (p<0.05), liver cirrhosis (p<0.01), and hepatoma (p<0.01). We concluded that there were significant decrease in the level of HDL2, in patients with chronic active hepatitis, liver cirrhosis, hepatoma and these are probably due to change in apolipoprotein and decreased activity of lipoprotein lipase.