The experimental study was carried out to assess the effect of trithiozine on gastric secretion in rats with the use of Shield rat method. The results were obtained as follows. 1. The volume of basal gastric secretion was 0. 3 cc/hr. in control group, and 0. 2 cc/hr. in trithiozine group. 2. The basal acid secretion was 0.44 Eq/hr. in control group, and 0.28 Eq/hr. in trithiozine group. The volume of stimulated gastric secretion was 0. 67 cc/hr. in control group, and 0.33cc/ hr. in histalog and trithiozine group. 4. The stimulated acid secretion was 2. 7 uEq/hr. in control group, and 0. 95 uEg/hr. in histalog and trithiozine group.