정상 혈압을 유지하고 있는 건강한 청년을 대상으로 furosemide 이뇨기간중과 이뇨후 1일간의 aldosterone 분비율, 배설율 및 혈중농도를 측정하여 아래와 같은 성적을 얻었다. 1) Furosemide 투여후 2시간 동안의 aldosterone 배설량은 투여를 받지 않은 사람에 비하여 현저히 증가하였다. 2) Furosemide 투여후 뇨중으로의 Na 및 K 배설량은 모두 현저히 증가하였다. 3) Furosemide 이뇨기간중의 뇨증 배설 증가량은 심히 증가되었으나 aldosterone 분비율은 훨씬 미급하였다. 4) Furosemide 이뇨후 1일간의 aldosterone 배설은 별다른 변동을 보이지 않았고 분비율 변동도 크지는 않았지만 1일동안의 총분비량 증가분은 이뇨로 잃은양을 보충할만한 분량이었다. 5) 이상 성적으로 보아 aldosterone 분비를 증가하는데 양적 한계가 있는 것으로 보였으나 이를 해명하기 위하여는 대사성 제거율 측정이 있어야 할 것으로 고찰되었다.

Marked augmentation of urinary aldosterone excretion following furosemide adnainistration was observed in previous experiment. In this study, author measured the changes of aldosterone secretion after furosemide administration in normotensive young volunteers with high sodium intake. After intraveeous injection of 1.2-3H-aldosterone, urine samples were collected in course of time until 24 hours after the injection. Furosemide administration was done at 30 minutes prior to aldosterone injection. Specific activities of 3H-aldosterone during and after diuresis were measured and aldosterone secretion rates were calculated dividing the doses by specific activities. Results were as followed. 1) Furosemide resulted in a marked increase in urinary aldosterone excretion. 2) Furosemide lead to an increase in both sodium and potassium excretion. 3) Aldosterone secretion rate was also inc rease d during furosemide diuresis, but the rate was smaller than that of urinary excretion. 4) Continuous modest increase in aldosterone secretion rate was shown after diuresis and tota1 excess amount of aldosterone secretion for 24 hrs was equivalent to the amount of aldosterone excretion produced by diruesis. 5) Abrupt marked loss of circulating aldosterone produced by diuresis was supplemented by long lasting increase in secretion for over twenty four hours.