Authors analysed biochemical studies and scintigraphic findings of obstructive jaundice and nonobstructive jaundice in 44 cases of Tc-99m-DISIDA scintigraphy with nonvisualization of biliary excretion till 120 min or 240 min after injection of Tc-99m-DISIDA. Causative diseases of Tc-99m-DISIDA scintigraphy with nonvisualization of biliary excretion were in order to choledocholithiasis (25%), hepatitis (25%), cholangiocarcinoma (14%), cholangitis (14%) and pancreas head tumor (11%). In obstructive jaundice, statistically significant findings were elevated alkaline phosphatase above 300 IU/L on biochemical study and single lobe enlargement of the liver, irregular radioisotope uptake of the liver and concave indentation of the gall bladder fossa of the liver on scintigraphy. In nonobstructive jaundice, statistically significant findings were persistent renal excretion of Tc-99m-DISIDA and more increased uptake density of the heart than the liver on scintigraphy.