It is well known that Tc-99m-sulfur colloid or phytate hepatic scintigraphy is highly sensitive but not specific. Both Tc-99m-DISIDA and bilirubin have been shown to share the same anionic transport pathway in the liver. Hepatocellular carcinoma (HCC) retains the ability to produce bile but has marked limitation to excreting it resulting in accumulation of bile within the tumor cells. Based upon such a fact, Tc-99m-DISIDA hepatobiliary scintigraphy is used for the diagnosis of HCC. The present communication deals with our experience of DISIDA scintigraphic exploration of 9 cases of HCC in a retrospective way. We have made an observation on intensity of positive radionuclide accumulation in the cold area of HCC as it is demonstrated by phytate scintigraphy. In addition we have semiquantitatively analyzed time-activity pattern and the following results were obtained.(1) All of 9 cases showed an increased uptake of Tc-99m-DISIDA in delayed scintigrams. Of these 5 cases showed accumulation less than, 3 equal to, 1 more than the surrounding liver tissue. (2) The mean of the first appearing time of Tc-99m-DISIDA activity in tumoral region was 2 hours and 20 minutes. (3) DISIDA scintigraphy provides us with positive informations of diagnostic value.