The most important object of periodontal treatment is the perfect regeneration of destruc- ted periodontal tissue. The healing of periodontal lesion is affected by several cells & factors, which result in formation of long juntional epithelium, root resorption, bony ankylosis or connective tissue attachment. And ideal healing is enhanced by epithilial exclusion or periodontal ligament cell activation. In this investigation, I studied the effect of Zizyphus Fructus extract which enhances biologic activity4 collagen synthesis, on the chemotaxis 8z cell nature. The cells were obtained from interdental aiea & middle third area of the freshly extracted teeth for the orthodontic purpose. And they were fully incubated in a-MEM solution containing 100μg/ml penicillin & 100μg/ml streptomycin followed by 6 generation incubation. The test cells were collected by trypsin-EDTA R centrifuge in the fully incubated cells, counted by Hemacyotmeter, incbated 5X10/ml cells for 24 hours, re-incubated 24 hours in media containing natural extract and photographed. The cells were incubated for 4 hours in 48 well microchemotaxis chamber bisecting upper & lower chamber by 8pm pore polycar- bonate membrane coating 5mg/ml gelatin solution. The migrated cells in microscope were counted, which meaned cell chemotaxis activity. The study had shown that the morphology of cell was spindle-shaped as the control group, and the subextract test groups were not significantly different. In gingival fibroblasts, the chemotaxis effect of PDGF was statistically significant compared to control group. The Zizyphus Fructus extract was more or less enhanced chemotaxis effect and in lμg/ml conce- ntration the chemotaxis effect was slightly elevated compared with 10pg/ml concentration. But, among the subextracts, it was not significantly defferent. In PDL cells, the chemotaxis effect of PDGF in statistically significant, and the zizyphus Fructus extract had shown the enhanced effect. The effect was slightly higher in lμg/ml concentration than 10 g/ml concent- ration, and no significance among the subextracts.