목적: 인간 자궁경부암에서 annexin-I의 역할을 규명하기 위해 annexin -I의 발현과 암세포증식과의 상관관계를 조사하였다. 방법: 면역조직화학 (immunohistochemical) 염색법 과 Western blotting으로 자궁경부암 조직에서 annexin-I 발현의 정도와 분포를 조사하였다. 악성세포주인 SiHa와 HeLa 세포주를 tamoxifen, β-estradiol과 all-trans retinoic acid로 3일 또는 5일간 처리한 후 각 세포의 증식을 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT ) colorimetric assay로 측정하였고 동시에 유세포측정기(flow cytometry)로 annexin-I의 발현을 조사하였다. 결과: Annexin-1에 대한 면역조직화학 분석 결과 자궁경부암세포가 진한 과립상으로 염색되어 관찰되었으며, Western blotting 분석에서는 annexin-I의 발현이 정상이나 양성조직보다 암조직에서 증가하였다. 한편, tamoxifen, β-estradiol 또는 all-trans retinoic acid와 같은 세포증식 조절물질을 이용한 세포주 실험에서는, tamoxifen이나 β-estradiol로 SiHa와 HeLa 세포주를 증식시킨 뒤 annexin-I의 발현이 증폭되었고 all-trans retinoic acid에 의한 항증식 후 annexin-I의 발현이 감소되었다. 결론: Annexin-I의 발현은 정상조직보다 자궁경부암에서 증가되어 있었고 자궁경부암 세포주의 증식과 비례해서 annexin-I의 발현이 증가되었다

Objectives: To investigate the role of annexin-I in human cervical cancer, we evaluated the expression of annexin-I and the relation with the proliferation of cancer cells. Methods: By immunohistochemical analysis and the western blotting of annexin-I , we investigated the extent and distribution of the expression of annexin-I in cervical cancer tissues. After treating the human cancer cell lines ( SiHa and HeLa cell lines ) with tamoxifen, estradiol, and retinoic acid for 5 days to make the cells proliferate and antiproliferate, we measured the proliferation simultaneously with 3-(4,5- dimethyl thiazol- 2-yl)-2,5-diphenyl-tetr -azolium bromide (MTT) colorimetric assay and the expression level of annexin-I with flowcytometry. Results: In the immunohistochemical stains, a granular staining pattern involving the entire cytoplasm was more heavily observed in malignant lesions than in normals. In the western blotting, the antibodies against 35-kDa annexin-I appeared to react more strongly with the lysates of cancer tissues than normal and benign tissues. In SiHa and HeLa cell lines with tamoxifen and β- estradiol treatment, increased expressions of annexin-I were noted with correlated increased proliferation of cells, and with the treatments of all trans retinoic acid, decreased expressions of annexin-I were noted with correlated decreased proliferation of cells. Conclusions: The results suggest that the expression of annexin-I might correlate with cervical cancer than normal and the proliferation of cancer cells.