본 연구는 착상연구의 모델로서 실험적으로 원하는 환경을 설정할 수 있고 관찰이 용이한 장점을 보여주어, 과거에 2차원적인 단순배양용기에서 시행되었던 체외배양 수준을 한 단계 끌어올린 것으로 사료된다. 그러나 이것은 생체의 자궁내막세포와 같은 dynamic milieu가 결여되어 있어, 실제의 자궁내막과 유사한 체계를 이루는 배양체계를 어느 정도 수준까지 완성하려면 지속적인 연구가 필요할 것으로 사료된다.

Since implantation of the blastocyst into the endometrium involves interactions which are difficult to analyse in vivo, we used a reconstituted basal lamina matrix preparation [Matrigel] to examine the in vitro interactions of blastocyst attachment. Matrigel is a solubilized extract of basal lamina material from Engel-Holm-Swarm mouse tumor cells and contains laminin, collagen IV, heparin sulphate proteoglycan, and number of growth factors. Blastocysts were transferred to dishes previously coated with Matrigel. By 48h, all blastocysts had attached to the surface of Matrigel pad. Following adhesion to the Matrigel, the blastocysts maintained their 3-dimensional integrity in contrast to control. The embryos in Matrigel were not flattening and kept polarity and spherical shape during culture. The polar trophoblast invaded the Matrigel downward unlike the horizontal growth in control. This experiment model would be an aid for establishment of blastocyst implantation study.